UNESA students researched
Unesa. ac.id, SURABAYA—The Exact Research Student Creativity Program Team (PKM-RE) from the Faculty of Mathematics and Natural Sciences (FMIPA) Surabaya State University (UNESA) succeeded in obtaining funding from Belmawa, Ministry of Education and Culture of the Republic of Indonesia with research entitled "Potential of Bioactive Peptides from Local Food Cincalok as a Natural Antibiotic against Mycobacterium tuberculosis”.
The team consists of, Maharani Dyah Arumsari (S1 Chemistry, 2021), Ariij Hady Tsana (S1 Biology, 2021), Bilqis Imroatus Sholiha (S1 Biology, 2021), Firnanda Ayu Rizki (S1 Biology, 2022), Rihan Ali Abdillah (S1 Biology, 2022). Under the guidance of Dwi Anggorowati Rahayu, S.Si., M.Sc., and a number of alumni such as A. Misbakhus Sururi, Nur Anisa Rosyidah, Dhea Anggraeni from the Bachelor of Science in Chemistry study program, 2019.
“Cincalok or known as Kecalok is a typical West Kalimantan regional food made from fermented rebon shrimp. "The ingredients used are shrimp, salt, sugar and onion powder," said Maharani Dyah Arumsari as team leader.
He further explained that the function of adding salt is to select and inhibit pathogenic microbes, while sugar provides glucose which supports bacterial growth, then salt powder serves as a flavoring. "So fermentation is said to be successful if the texture of the shrimp becomes crushed, has a pinkish color, and has a sour taste and aroma," he added.
The fermentation that occurs in the process of making cincalok occurs naturally, the PKM Team uses lactic acid bacteria (LAB) such as Lactobacillus sp., Lactococcus lactis, and Streptococcus sp. These bacteria degrade carbohydrates into lactic acid. In addition, the protein content in rebon shrimp is broken down into bioactive peptides through hydrolysis and fermentation processes by LABLactococcus lactis.
“Our team also added the bacteria Lactococcus lactis subsp. "Lactis FNCC 008 in making cincalok food which aims to help the fermentation process occur optimally and improve the quality of cincalok," he said.
In this way, local Cincalok food contains bioactive peptides which have the potential to be a natural, safe and effective alternative treatment to fight Mycobacterium tuberculosis as the cause of TB disease," he added.
The team leader explained that this research involved various stages, starting from making cincalok, extracting bioactive peptides from cincalok, testing the quality of cincalok including calculating the number of lactic acid bacteria carried out using the Total Plate Count (TPC) method and pH measurement.In addition, an antimicrobial activity test was carried out in vitro which was compared with the drug control. Before the in vitro testing was carried out, the bioactive peptide compounds produced were identified using thin layer chromatography (TLC) and high performance chromatography (HPLC). , Supervisor Dwi Anggorowati Rahayu said that this research is very important considering the increasing resistance of TB bacteria to conventional antibiotics.
The use of bioactive peptides from cincalok as a natural alternative could be a solution in efforts to treat TB. Apart from that, this research shows how collaboration between science and local wisdom can produce innovations that have a broad impact.
"We hope that this research can contribute to improving the quality of local cincalok food so that it has health benefits and overcomes the problem of tuberculosis through the development of safer and more effective natural medicines.
Given that the death rate resulting from tuberculosis through Mycobacterium tuberculosis infection is the 2nd highest in the world and has a death toll of 1.13 million people, accounting for twice as many as "This is WHO data for HIV/AIDS deaths, 2023. Indonesia is ranked 2nd in tuberculosis cases in the world with 845,000 cases," he said.
The PKM-RE team is determined that their research can inspire other parties to continue to innovate in the future. improving the quality of local food and finding creative solutions to global health challenges. Where the UNESA PKM-RE Team will continue to study and explore data in more detail and carry out further research in the laboratory.[]
***
Author: UNESA PKM-RE Team/Muhammad Azhar Adi Mas'ud (FBS)
Photo: UNESA PKM-RE Team
Share It On:
